ZNF292

lncRNA

Cell Type Determination

HP1 Complex

Two protein-protein interaction databases, BioGrid^3.5 and IntAct, include interactions between ZNF292 and other proteins including HP-1 components CBX1, CBX3 and CBX5 (Huari 2016). They also found an interaction with SUV39H1, the H3-K9-HTMase. Proximity labeling places ZNF292 near HIST1H3A (Lui 2018).

Ostapcuk (2018) Studied the role of ADNP in recruiting HP1 and CHD4. In one of their experiments they immunoprecipitated FlagAviTag-tagged proteins from mouse ES cells. Tagged HP1α (CBX5), HP1β (CBX1) and HP1γ (CBX3) all coprecipitated ZNF292's nearest paralog RLF. HP1γ immunoprecipitated ZNF292 and WIZ - another widely-spaced zinc finger protein. The ADNP-HP1-CHD4 complex may be involved in maintaining distinct cellular states. ADNP like ZNF292 is a C2H2 zinc finger protein, but several of its zinc finger motifs are flagged as non-typical by ProSite Prorule annotation. ADNP also contains a C-terminal homeobox domain.

Protein	Associated complex	Function	Species	Method	Reference
SLAMF1	Membrane	Lymphocyte signaling	H. sapiens	Affinity Capture-MS	Huttlin, EL 2015
P4HB	Cell surface	Disulfide-isomerase	H. sapiens	Affinity Capture-MS	Huttlin, EL 2015
YEATS4	NuA4 histone acetyltransferase	H2A,H4 acetylation	H. sapiens	Affinity Capture-MS	Hien, MY 2015
NEDD1	Mitotic spindle	Nucleation of microtubules from the spindle	H. sapiens	Affinity Capture-MS	Hien, MY 2015
MTA2	Nucleus	?	M. musculus	Affinity Capture-MS	Hien, MY 2015
NAA50	Nucleus	N-alpha-acetyltransferase	M. musculus	Affinity Capture-MS	Hien, MY 2015
ERP44	Endoplasmic reticulum	Protein disulfide isomerase	M. musculus	Affinity Capture-MS	Hien, MY 2015
CBX1	HP-1	function	H. sapiens	Affinity Capture-MS	Hauri, S 2016
CBX3	HP-1	function	H. sapiens	Affinity Capture-MS	Hauri, S 2016
CBX5	HP-1	function	H. sapiens	Affinity Capture-MS	Hauri, S 2016
SUV39H1	HP-1	Histone-lysine N-methyltransferase	H. sapiens	Affinity Capture-MS	Hauri, S 2016
ESR2	Nucleus	Estrogen receptor beta	H. sapiens	Affinity Capture-MS	Giurato, G 2018
SOX2	Nucleus	Transcription factor	H. sapiens	Proximity Label-MS	Kim, BR 2017
HIST1H3A	HP-1	Nucleosome core component	H. sapiens	Proximity Label-MS	Liu, X 2018
KIAA1429	WMM, nucleus	m6A methylation of RNA	H. sapiens	Affinity Capture-RNA	Yue, Y 2018

Zinc finger proteins interacting with HP-1 from Huari et. al. (2016)

Gene	Known and possible PPI domains	PPIs of interest
WIZ	CTBP1, CTBP2 ppi PLNLT 1030-1034, PLNLS 1214-1218	EHMT1, EHMT2
ZNF644
ZNF462	PPVSL 208	PBX1
ZNF518A	PVVVL 1346
ZMYM4	PIVLL 1376
ZRAB2
ZMYM2
ZNF280C
ADNP2	PSVLL 1107, Homeobox 1043-1102
ZSA5A	SCAN 44-126
ZMYM3	PFVLL 1205
ADNP	PGVLL 820, Neuroprotective peptide 354,361, Homeobox 754-814
ZNF518B	PSVAL 477
ZNF689	KRAB 29-100
POGZ	HTH CENPB 1015-1085, DDE-1 1117-1323, CBX5 ppi 810-850	CBX1,3&5
ZNF292	PHVTL 586, PTSVL 2690
RLF	PVVVL 1884
ZNF678
ZNF280D
ZNF581

ILC3 Cells

Lui (2017) studied the role of lncKdm2b in the maintenance of ILC3 cells, a key cell in the intestinal response to bacterial infections. They found deletion of lncKdm2b greatly reduced the number of ILC3 cells in the intestines of mice and made them more susceptible to bacterial infections such as Citrobacter Rodentium. As part of their investigation they found that lncKdm2b trans activates ZNF292 by recruiting transcriptional components to it’s promoter. They also reported a ZNF292 -/- mouse which showed sharply reduced ICL3 population. Of interest in the research is the implication that ZNF292 has a role in maintenance of at least one cell type and a mouse ZNF292-/- mouse is viable making it a potnetial model for studying ZNF292 haploinsufficiency's role in neurodevelopmental disorders.